Two methods are described for the simultaneous determination of Moxifloxacin HCl (MOX) and Bromfenac Sodium (BROM) in binary mixture. The first method was based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 260nm. The separation was carried out on Merck HPTLC aluminum sheets of silica gel 60F254 using Methylene chloride: Acetonitrile: Methanol: Ammonia (2.5:2.5:2.0:1.0 v/v/v/v) as mobile phase. The linear regression analysis data was used for the regression line in the range of 108–756ng/spot and 600–4200ng/spot for BROM and MOX, respectively. The second method was based on HPLC separation of the two drugs on the reversed phase ACE column [C18(5μm,150mm×4.6mm,i.d.)] at ambient temperature using a mobile phase consisting of 10mM phosphate buffer pH 7.0 and Acetonitrile (72:28,v/v) and flow rate was 1.0ml/min. Quantitation was achieved with UV detection at 265nm based on peak area with linear calibration curves at concentration ranges 1.8–18μg/ml and 10–100μg/ml for BROM and MOX, respectively. Both methods were validated in terms of precision, robustness, recovery and limits of detection and quantitation.The analysis of variance (ANOVA) and Student’s t-test were applied to correlate the results of BROM and MOX determination in dosage form by means of HPTLC and HPLC method.
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